human liver hepatocellular carcinoma cell line hep g2  (ATCC)

Journal: BMC Gastroenterology

Article Title: Elevated CircYthdc2 expression is correlated with aggressive features and poor progression-free survival in hepatocellular carcinoma

doi: 10.1186/s12876-025-04231-0

Figure Lengend Snippet: YTHDC2 methylation and survival analysis in LIHC. A Analysis of YTHDC2 promoter methylation levels in 377 LIHC tissues compared to 50 normal liver tissues using the UALCAN database, showing significant hypomethylation in LIHC tissues. B Analysis of YTHDC2 expression levels in 371 LIHC tissues compared to 50 normal liver tissues using the UALCAN database, showing significant upregulation in LIHC tissues

Article Snippet: Human liver hepatocellular carcinoma cell lines (Bel-7402 and PLC/PRF/5) and normal human liver cell line L02 (American Type Culture Collection) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (Gibco) at 37 °C with 5% CO 2 in a humidified incubator.

Techniques: Methylation, Expressing

Journal: BMC Gastroenterology

Article Title: Elevated CircYthdc2 expression is correlated with aggressive features and poor progression-free survival in hepatocellular carcinoma

doi: 10.1186/s12876-025-04231-0

Figure Lengend Snippet: CircYTHDC2 expression analysis across multiple LIHC models. A RT-qPCR analysis of circYTHDC2 expression in 72 paired LIHC tissues and adjacent normal tissues ( P < 0.001). B Serum circYTHDC2 levels measured by RT-qPCR in 72 LIHC patients, 40 cirrhosis patients and 72 healthy controls. C CircYTHDC2 expression levels in LIHC cell lines (Bel-7402 and PLC/PRF/5) compared to normal human liver cell line L02. *** P < 0.001

Article Snippet: Human liver hepatocellular carcinoma cell lines (Bel-7402 and PLC/PRF/5) and normal human liver cell line L02 (American Type Culture Collection) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (Gibco) at 37 °C with 5% CO 2 in a humidified incubator.

Techniques: Expressing, Quantitative RT-PCR

Journal: BMC Gastroenterology

Article Title: Elevated CircYthdc2 expression is correlated with aggressive features and poor progression-free survival in hepatocellular carcinoma

doi: 10.1186/s12876-025-04231-0

Figure Lengend Snippet: Diagnostic potential and stability analysis of circYTHDC2. A ROC curve analysis of tissue circYTHDC2 expression for LIHC diagnosis (AUC: 0.846, 95% CI: 0.775–0.914; sensitivity: 82.4%, specificity: 76.8%). B ROC curve analysis of serum circYTHDC2 levels (AUC: 0.788, 95% CI: 0.703–0.873). C RNase R resistance assay in Bel-7402 cells showing maintained circYTHDC2 levels compared to linear YTHDC2 mRNA after RNase R treatment. D RNase R resistance assay in L02 cells demonstrating similar stability characteristics of circYTHDC2

Article Snippet: Human liver hepatocellular carcinoma cell lines (Bel-7402 and PLC/PRF/5) and normal human liver cell line L02 (American Type Culture Collection) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (Gibco) at 37 °C with 5% CO 2 in a humidified incubator.

Techniques: Diagnostic Assay, Expressing, Biomarker Discovery

Journal: BMC Gastroenterology

Article Title: Elevated CircYthdc2 expression is correlated with aggressive features and poor progression-free survival in hepatocellular carcinoma

doi: 10.1186/s12876-025-04231-0

Figure Lengend Snippet: Survival analysis based on circYTHDC2 expression levels. A Kaplan-Meier analysis of progression-free survival in LIHC patients stratified by circYTHDC2 expression levels. B Overall survival analysis comparing high and low circYTHDC2 expression groups. Patients were stratified based on median circYTHDC2 expression level (13.41). Risk tables below each curve indicate the number of patients at risk at 0, 10, 20, and 30 months of follow-up

Article Snippet: Human liver hepatocellular carcinoma cell lines (Bel-7402 and PLC/PRF/5) and normal human liver cell line L02 (American Type Culture Collection) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (Gibco) at 37 °C with 5% CO 2 in a humidified incubator.

Techniques: Expressing

Journal: BMC Gastroenterology

Article Title: Elevated CircYthdc2 expression is correlated with aggressive features and poor progression-free survival in hepatocellular carcinoma

doi: 10.1186/s12876-025-04231-0

Figure Lengend Snippet: YTHDC2 stabilizes circYTHDC2 via m6A modification. A Pearson correlation analysis was used to assess the correlation between the expression of YTHDC2 and circYTHDC2 in the tumor samples from LIHC patients ( n = 72). B qRT-PCR analysis was conducted to determine the overexpression efficiency of YTHDC2 in Bel-7402 and PLC/PRF/5 cells. C qRT-PCR analysis was used to measure the expression of circYTHDC2 in Bel-7402 and PLC/PRF/5 cells after YTHDC2 overexpression. D The impact of YTHDC2 overexpression on the RNA stability of circYTHDC2 was measured in Bel-7402 and PLC/PRF/5 cells with ActD treatment for indicated time. E RIP assays were used to detect the binding between anti-m6A with circYTHDC2 in Bel-7402 and PLC/PRF/5 cells after YTHDC2 overexpression. F RIP asssays were performed to explore the interaction between circYTHDC2 and YTHDC2 in Bel-7402 and PLC/PRF/5 cells. G RNA-Pull down assays were used to determine the binding between circYTHDC2 and YTHDC2 in Bel-7402 and PLC/PRF/5 cells. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Human liver hepatocellular carcinoma cell lines (Bel-7402 and PLC/PRF/5) and normal human liver cell line L02 (American Type Culture Collection) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (Gibco) at 37 °C with 5% CO 2 in a humidified incubator.

Techniques: Modification, Expressing, Quantitative RT-PCR, Over Expression, Binding Assay

Journal: Discover Oncology

Article Title: Isosilybin B: a potential novel therapeutic agent with hepatoprotective, anticancer and antifibrotic properties

doi: 10.1007/s12672-025-03380-8

Figure Lengend Snippet: Effect of IB/SB/SM on cytotoxicity and cell cycle arrest of liver cells. A : Chemical structure of IB and SB (silybin A and silybin B in ratio 1:1). Cytotoxicity in Hepa1-6 ( B ), HepG2 ( C ) or AML12 ( D ) liver cells after 24 h treatment with IB/SB/SM. Changes in the cell cycle in Hepa1-6 ( E ), HepG2 ( F ), or AML12 ( G ) liver cells after 24 h treatment with 31.3 µg/mL of IB/SB/SM. H : Cell distribution of diploid cells in the phases of the cell cycle shown for IB (31.3 µg/mL). The data are given as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Human liver hepatocellular carcinoma cell line HepG2 (RRID: CVCL_0027, ATCC, USA) was cultivated in RPMI medium supplemented with 10% FBS and 1% penicillin-streptomycin.

Techniques:

Journal: Research in Pharmaceutical Sciences

Article Title: Synthesis and in vitro evaluation of self-assembling biocompatible heparin-based targeting polymeric micelles for delivery of doxorubicin to leukemic cells

doi: 10.4103/RPS.RPS_197_24

Figure Lengend Snippet: Fluorescent and visible light microscopy images of K562 (rows 1 and 2) and HepG2 (rows 3 and 4) cells incubated with culture medium, free DOX, DOX/HEP-DEX, and DOX/FA-HEP-DEX micelles after 4 h incubation. DOX, Doxorubicin; FA, folic acid; HEP, heparin; DEX, dexamethasone.

Article Snippet: Human erythroleukemic (K562) and human liver hepatocellular carcinoma (HepG2) cell line s were supplied by the Pasteur Institute of Iran (Iran, Tehran).

Techniques: Light Microscopy, Incubation

Journal: Research in Pharmaceutical Sciences

Article Title: Synthesis and in vitro evaluation of self-assembling biocompatible heparin-based targeting polymeric micelles for delivery of doxorubicin to leukemic cells

doi: 10.4103/RPS.RPS_197_24

Figure Lengend Snippet: The cellular uptake percentage of free DOX, DOX/HEP-DEX, and DOX/FA-HEP-DEX micelles after 4 h incubation in K562 and HepG2 cells. DOX, Doxorubicin; FA, folic acid; HEP, heparin; DEX, dexamethasone.

Article Snippet: Human erythroleukemic (K562) and human liver hepatocellular carcinoma (HepG2) cell line s were supplied by the Pasteur Institute of Iran (Iran, Tehran).

Techniques: Incubation

Journal: Research in Pharmaceutical Sciences

Article Title: Synthesis and in vitro evaluation of self-assembling biocompatible heparin-based targeting polymeric micelles for delivery of doxorubicin to leukemic cells

doi: 10.4103/RPS.RPS_197_24

Figure Lengend Snippet: In vitro cytotoxicity of free DOX, DOX-loaded micelles, and blank micelles evaluated against (A) K562 cells after 48 h incubation, (B) K562 cells after 72 h incubation, (C) HepG2 cells after 48 h incubation, and (D) HepG2 cells after 72 h incubation. Data are plotted as the mean ± SD, n = 3. DOX, Doxorubicin; FA, folic acid; HEP, heparin; DEX, dexamethasone.

Article Snippet: Human erythroleukemic (K562) and human liver hepatocellular carcinoma (HepG2) cell line s were supplied by the Pasteur Institute of Iran (Iran, Tehran).

Techniques: In Vitro, Incubation

Journal: Research in Pharmaceutical Sciences

Article Title: Synthesis and in vitro evaluation of self-assembling biocompatible heparin-based targeting polymeric micelles for delivery of doxorubicin to leukemic cells

doi: 10.4103/RPS.RPS_197_24

Figure Lengend Snippet: IC 50 values of DOX from free DOX, DOX/FA-HEP-DEX and DOX/HEP-DEX micelles after 48 h and 72 h exposure to K562 and HepG2 cells. Data represent mean ± SD, n = 3. ** P < 0.01 and *** P < 0.001 represent significant differences in comparison with free DOX; ## P < 0.01 versus DOX/HEP-DEX.

Article Snippet: Human erythroleukemic (K562) and human liver hepatocellular carcinoma (HepG2) cell line s were supplied by the Pasteur Institute of Iran (Iran, Tehran).

Techniques: Comparison

Journal: BMC complementary medicine and therapies

Article Title: Effects of essential oil of Origanum onites and its major component carvacrol on the expression of toxicity pathway genes in HepG2 cells.

doi: 10.1186/s12906-024-04571-6

Figure Lengend Snippet: Fig. 2 Inhibition effects of DNA synthesis from the EOOO. (0.02–0.2 µg/mL) (A) and CV (7.5–105 µg/mL) (B) on the growth of HepG2 cells after 48 h. The EOOO and CV induced inhibition of DNA syntheses were in a concentration-dependent manner by the method BrdU incorporation assay. All values are expressed as mean ± SD at least three separate experiments performed in quadruplicate. Differences were considered significant compared to the control group from *p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001

Article Snippet: A human liver hepatocellular carcinoma cell line HepG2 purchased from DSMZ (Braunschweig, Germany) was cultured in DMEM (Dulbecco Modified Eagle Medium) (Sigma) supplemented with 10% FBS (fetal bovine serum) (PAA Lab. GmbH, Les Mureaux, France), penicillin/ streptomycin at 100 units/ml and 2 mM L-glutamine as adherent monolayers.

Techniques: Inhibition, DNA Synthesis, Concentration Assay, BrdU Incorporation Assay, Control

Journal: BMC complementary medicine and therapies

Article Title: Effects of essential oil of Origanum onites and its major component carvacrol on the expression of toxicity pathway genes in HepG2 cells.

doi: 10.1186/s12906-024-04571-6

Figure Lengend Snippet: Fig. 3 Effects of the EOOO (A) (0.08 and 0.09 µg/mL) and (B) CV (45 and 75 µg/mL) on viability of HepG2 cells incubated for 24, 48, 72 and 96 h. Each value is the mean ± S.D. of three separate experiments performed in quadruplicate. Differences were considered significant compared to the control group from *p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001

Article Snippet: A human liver hepatocellular carcinoma cell line HepG2 purchased from DSMZ (Braunschweig, Germany) was cultured in DMEM (Dulbecco Modified Eagle Medium) (Sigma) supplemented with 10% FBS (fetal bovine serum) (PAA Lab. GmbH, Les Mureaux, France), penicillin/ streptomycin at 100 units/ml and 2 mM L-glutamine as adherent monolayers.

Techniques: Incubation, Control